Evaluation of the stromal vascular fraction of adipose tissue as the basis for a stem cell-based tissue-engineered vascular graft
نویسندگان
چکیده
منابع مشابه
Tissue Dissociation Enzymes for Adipose Stromal Vascular Fraction Cell Isolation: A Review
With the increasing clinical translation of adipose stromal cell based technologies, it is important to thoroughly report on all aspects of these technologies. In this article, we review the commonly used enzymes for adipose tissue dissociation and stromal vascular fraction isolation. The enzymes reviewed include collagenase, trypsin, clostripain, and the neutral proteases dispase and thermolys...
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One of the major challenges in cell implantation therapies is to promote integration of the microcirculation between the implanted cells and the host. We used adipose-derived stromal vascular fraction (SVF) cells to vascularize a human liver cell (HepG2) implant. We hypothesized that the SVF cells would form a functional microcirculation via vascular assembly and inosculation with the host vasc...
متن کاملShort-chain fatty acids enhance adipocyte differentiation in the stromal vascular fraction of porcine adipose tissue.
BACKGROUND Short-chain fatty acids (SCFAs), including acetate, propionate, and butyrate, are the main products of microbial fermentation in the gut and might mediate some of the effects of gut microbiota and nutrition on development, metabolism, and pathogenesis of obesity and other diseases. OBJECTIVE The objective of this study was to determine the effects of SCFAs on adipocyte differentiat...
متن کاملAdipose tissue stromal vascular fraction in the treatment of full thickness burns in rats.
PURPOSE: To analyze the healing effects of stromal vascular fraction (SVF) application compared to wound dressing with 2% silver sulfadiazine in full thickness burn wounds in rats. METHODS: Animals were divided into two groups: 2% silver sulfadiazine group and SVF group. Both groups received occlusive bandages while the first one was treated with 2% silver sulfadiazine and the latter was tr...
متن کاملChondrocytes Cocultured with Stromal Vascular Fraction of Adipose Tissue Present More Intense Chondrogenic Characteristics Than with Adipose Stem Cells.
Partial replacement of chondrocytes by stem cells has been proposed to improve the performance of autologous chondrocyte implantation (ACI). Our previous studies showed that the increased cartilage production in pellet cocultures of chondrocytes and mesenchymal stem cells (MSCs) is due to a trophic role of the MSCs by stimulating chondrocyte proliferation and matrix production rather than MSCs ...
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ژورنال
عنوان ژورنال: Journal of Vascular Surgery
سال: 2017
ISSN: 0741-5214
DOI: 10.1016/j.jvs.2016.09.034